Biodegradation of Phenol by Bacillus simplex: Characterization and Kinetics Study

Phenol is one of the main pollutants that have a serious impact on environment and can even be very critical to human health. The biodegradation phenol considered an increasingly important pollution control process. In this study, degradation by Bacillus simplex was investigated for first time under different growth conditions. Six initial concentrations were used as primary substrate. Culture conditions had effect these cells' ability biodegrade phenol. best organism its highest level noticed at pH 7, temperature 28 °C, periods 36 96 h, respectively. GC-MS analysis bacterial culture sample revealed further catechol 1,2-dioxygenase produce cis, cis-mucconic acid via ortho-pathway and/or 2,3-dioxygenase into 2-hydroxymucconic semialdehyde meta-pathway. rate perceived 700 mg/L concentration. Approximately 90% (700 mg / L) removed in less than hours incubation time. It found Haldane model fitted relationship between specific concentration, whereas profiles with could adequately described modified Gompertz model. obtained parameters from equation are: 1.05 h−1, 9.14 ppm, 329 ppm Haldane's maximum rate, half-saturation coefficient, Haldane’s kinetics inhibition experimental data minimizing sum squared error (SSR) 1.36 X 10-3.